DISCVR-Seq Genome Requirements:

Any genome FASTA files used with DISCVR-seq tools must be indexed with samtools faidx, and have a sequence dictionary created by Picard's CreateSequenceDictionary. These requirements are identical to most tools from GATK and Picard.

See here for more information on the purpose of the index/dictionary files.

Below are example commands to create these files:

    samtools faidx reference.fasta

    java -jar picard.jar CreateSequenceDictionary \
        R=reference.fasta \
        O=reference.dict